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KMID : 0613820110210091266
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Journal of Life Science
2011 Volume.21 No. 9 p.1266 ~ p.1273
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Effect of Saponin with Antioxidant Activity on Matrix Metalloproteinase in Human Dermal Fibroblasts
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Park Hye-Jung
Kim Moon-Moo
Lee Dong-Hwan
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Abstract
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Saponin is a main component of ginseng widely known as an oriental traditional medicinal ingredient. A variety of biological effects of saponin has been reported, but its action related to skin regeneration has remained unclear so far. In this study, the effect of saponin on matrix metalloproteinase as well as its antioxidant effect in cell free system was examined in human dermal fibroblasts. First of all, as a result of investigating the effect of saponin on cell viability using MTT assay, it was shown to increase cell viability below 10 μg/ml, but it also showed cytotoxicity above 25 μg/ml. The antioxidant effect of saponin was exerted by inhibition of H₂O₂ in addition to reducing power above 1 μg/ml. In particular, saponin showed a protective effect on DNA oxidation. Furthermore, it was observed that saponin activates MMP-2 and increases MMP-1 activity in gelatin and casein zymography analyses, respectively, indicating that saponin could have potential a therapeutic agent for anti-aging and skin regeneration.
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KEYWORD
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Saponin, skin regeneration, human dermal fibroblasts, ROS, matrix metalloproteinase
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